GELATINASE PRODUCTION POTENTIAL OF BACTERIA ISOLATED FROM ATIMBO AND KWA RIVER SEDIMENTS



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GELATINASE PRODUCTION POTENTIAL OF BACTERIA ISOLATED FROM ATIMBO AND KWA RIVER SEDIMENTS



ABSTRACT

 

            The industrial importance of gelatinase cannot be overemphasized as more jaw dropping discoveries have uncovered the various undisputable applications of the enzyme in several aspects of science. In this study, gelatinase producing bacteria were probed from sediment samples of Kwa River and Atimbo River, Cross River State. Primary screening uncovered 3 isolates (B5, A7 and B6) that could elaborate gelatinase activity. This exposed the quantity of gelatinolytic bacteria to be 5.4% of the entire sediment sample population. The isolates that showed positive gelatinolytic activity were then quantitatively screened using the Bradford method which displayed high production in 2 isolates (B6 and B5).  The isolate that showed maximum gelatinolytic activity was then centrifuged and evaluated to determine the extracellular production of the enzyme using cell free supernatants. The cell free supernatants of positive isolates were used as crude gelatinase. Determination of carbon source required by the bacteria for optimal production of the gelatinase showed high rates of enzyme activity in sucrose which was selected. The successful organism was identified to be of Bacillus species using identification tests.

 

TABLE OF CONTENTS

 

Title page                                                                                                       i

 

Certification                                                                                                   ii

 

Dedication                                                                                                      iii

 

Acknowledgements                                                                                       iv

 

Abstract                                                                                                         v

 

Table of Contents                                                                                          vi

 

List of Figures                                                                                               vii

 

List of Plates                                                                                                  viii

 

List of Tables                                                                                                 ix

 

CHAPTER ONE: INTRODUCTION                                                        1

 

Aim and objectives of study                                                               3

Justification of study                                                                          3

 

 

CHAPTER TWO: LITERATURE REVIEW                                          4

 

Gelatins                                                                                               4

Sources of gelatin                                                                               7

Production of gelatin                                                                          9

Gelatin structure                                                                                 12

Mechanism of gelatin gelation                                                            14

Properties of gelatin                                                                            15

Industrial applications of gelatin                                                        17

Proteases                                                                                             21

Microbial proteases                                                                             22

Applications of microbial proteases                                                    24

Matrix Metalloproteases (MMPs)                                                       27

Gelatinase                                                                                           29

Types of gelatinases                                                                            30

Structure of gelatinases                                                                       31

Activation of gelatinases                                                                    32

Initiation of gelatinases                                                                       33

CHAPTER THREE: MATERIALS AND METHODS                          34

 

Materials                                                                                             34

Methods                                                                                              34

Sampling                                                                                             34

Sample collection                                                                                  34

Sample preparation                                                                               35

Microbial analysis                                                                                 35

Enumeration                                                                                          36

Isolation                                                                                                36

Primary screening for gelatinase-liquefying bacteria                 36

Secondary screening for gelatinase production in submerged culture  37

Evaluation of carbon sources for optimal gelatinase production           39

Characterization and identification of selected

gelatinase-producing bacterium                                                             41

 

CHAPTER FOUR: RESULTS                                                                      42

 

Microbial analysis                                                                                  42

Primary screening for gelatinase-liquefying bacteria                            42

Secondary screening for gelatinase production in submerged culture  43

Evaluation of carbon sources for optimal gelatinase production          43

Characterization and identification of selected

gelatinase-producing bacterium                                                              44

 

CHAPTER FIVE: DISCUSSION, CONCLUSION AND RECOMMENDATION                                                                                                                     49

 

Discussion                                                                                           49

Conclusion                                                                                          50

Recommendation                                                                                51

References 

Citation - Reference

All Project Materials Inc. (2020). GELATINASE PRODUCTION POTENTIAL OF BACTERIA ISOLATED FROM ATIMBO AND KWA RIVER SEDIMENTS. Available at: https://allprojectmaterials.com/department/paper-8410.html. [Accessed: ].

GELATINASE PRODUCTION POTENTIAL OF BACTERIA ISOLATED FROM ATIMBO AND KWA RIVER SEDIMENTS


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